circRNA研究概述

latory potency." Nature 495(7441): 333-338.РZhang, X. O., et al. (2014). "Complementary sequence-mediated exon circularization." Cell 159(1): 134-147.РZhang, Y., et al. (2013). "Circular intronic long noncoding RNAs." Molecular Cell 51(6): 792-806.РcircRNA的研究方法?---knockdownРLi, Z., et al. (2015). "Exon-intron circular RNAs regulate transcription in the nucleus." Nature Structural & Molecular Biology 22(3): 256-264.РNuclear run-on experimentsРKnockdown;?Extracting cell nuclei for run-on experimentsРThe siRNA oligo target circRNA junctionРcircRNA环化原理РChen, L. L. and L. Yang (2015). "Regulation of circRNA biogenesis." RNA Biology 12(4): 381-388.Р汉恒自主研发的超强成环系统Р体内转录Р剪接环化Р成环Р汉恒HB circ-EnhancedTM circRNA环化系统Р汉恒HB circ-EnhancedTM circRNA研究工具载体系统РLentilvirusРAdenovirusРAAVР细胞系Р细胞系、原代细胞、器官Р靶向特异性组织器官