富钒鹰嘴豆芽对糖尿病大鼠糖脂代谢及学习记忆的影响

tenciesandswimmingpathswererecordedusingthevideotrackingsystemforeachrat。3(2).InordertoinvestigatethechangesofhippocampalneuronsandtheeffectofVCS,electronmicroscopeandimmunohistochemistrywereused.ThenthehippocampusmRNAwasthenextracted,andcorrespondingeDNAwassynthesized.RealTime-PCRwasusedtodetectmRNAexpressionsofamyloidproteinprecursor(App),andCaspase3.Atthesametime,thebrainwassliced,andimmunoblotting(westem-blotting)wasusedtodetecttheproteinexpressionsofCaspase3,bax,bcl一2,IGF一1,andIGF-1R.4.StatisticalanalysisOne—wayanalysisofvariance(ANOVA)paredtheMeans+SEM.CorrelationanalysisWasmadeusingSpearmananalysis.ThesignificancelevelsweretestedatP<0.05.Results2(1).After9dfromtheadministrationwithSTZ(45mg/kg,i.p.),thediabeticratshaddiabeticsymptomssuchaspolydipsia,polyuria,andhyperglycemia.Andafter