4mLof0.72%Fibrinogensolutionintoatesttube,andpreincubateitina37±0.3℃waterbathfor5minutes.9.Add0.1mLofThrombinsolutionandmix.10.Afterexactly10minutes,add2mLof0.2mol/LTCAsolutionandmixfor5seconds.11.Add0.1mLofsampleintosolution,mixfor5seconds,andincubateat37±0.3℃for20minutes.12.Transferthemixtureintoamicrotesttubeandcentrifugeat15000rpmfor5minutes.13.Transferthe1mLofsupernatantcarefullyintoacuvettebyPasteurpipetteandreadtheabsorbance(AB)at275nm.(4)CALCULATIONNattokinaseactivity(FU/g)=(AT-AB)/0.01×1/60×1/0.1×D D:DilutionrateofsampleNotesⅠ.Themeasuredactivitycanvarywiththelotoffibrinogen.Accordingly,whenfibrinogen’slotischanged,correctthemeasuredactivitybymultiplyingthecorrectionfactorifnecessary.Ⅱ.Thisdiluentcanbekept15daysattheroomtemperature.Ⅲ. Usemixerforeverymixing.Ⅳ.uracyofthetestresult.
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