免疫印迹总蛋白内参定量与免染技术

50–254.VigelsøA,DybboeR,,DelaF,HelgeJW,GuadalupeGrauA.GAPDHandβ-actinproteindecreaseswithaging,making StainFreetechnologyasuperiorloadingcontrolinWesternblottingofhumanskeletalmuscle.JApplPhysiol(2015)118(3):386-94.总蛋白线性更宽,paringcellsreceiveddifferentchemicalordrugtreatmentthatmaycausechangesonHKPs--kinaseinhibitortreatmentNeurobiologyofDisease54(2013)280–288Whencellbehaviorandmorphologymaychangedramaticallyinatimecoursestudy–cellcyclestudyJ.Biol.Chem. 2011 286: 37483-37495.icmodifications–transformation,transfection,transgenicmodels,KnockoutsTHEJOURNALOFBIOLOGICALCHEMISTRYVOL.287,NO.6,pp.3733–3750,February3,2012THEJOURNALOFBIOLOGICALCHEMISTRYVOL.286,NO.35,pp.30662–30669,September2,20112010-2015年,超过200篇JBC文章使用了总蛋白代替看家蛋白作为WB内参!总蛋白线性更宽,表达量相对恒定9JBC推荐使用TotalProtein做归一化10Nature对WesternBlot数据的要求