tro metabolism of AEEРMethod:?1. Dog liver microsomes were prepared by differential centrifugation.?2. the protein concentrations of dog liver microsomes were measured using BSA method(9.5-10.1mg·mL-1).?3. The content of CYP450 in liver microsome were measured with CO differential spectroscopy (0.18 nmol·mg-1 ).?4. liver microsome enzyme activity was measured by coumarin transforming into 7-hydroxycoumarin.?5. In vitro incubation system was established, including Tris-HCl 0.05mol·L-1, liver microsome 0.6mg·mL-1 , MgCl2 5mmol·L-1 , 6-phosphate glucose 10 mmol·L-1 , NADP 2 mmol·L-1 , 6-phosphate glucose dehydrogenase 5 U·mL-1,total volume 400 μL.РLiu, et al., 2009; Smith, et al., 1985РcoumarinР7- hydroxycoumarinРCYP has activityРcoumarin transformed into 7-hydroxycoumarinРSample ?preparation
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