tmentofEtiology&Carcinogenesis,CancerInstituteandHospital,ChineseAcademyofMedicalSciences,Beijing100021,China;2)ChinaNationalCenterforBiotechnologyDevelopment,Beijing100036,China)AbstractChromosomeconformationisthoughttoplayanimportantroleingeneexpressionregulation.Chromosomeconformationcapture(3C),circularchromosomeconformationcapture(4C),3C-carboncopy(5C),ChIP-loopassay,ChIA-PETandHi-anizationoflonggenomicregionsinlivingcells.Allstepsof3C,those3C-basedmethodsandkeyproceduresinexperimentwerealsodiscussed.Theaimofthispaperistointroducesomenewmethodstostudygeneregulationin3D.Keywordschromosomeconformation,chromosomeconformationcapture,generegulation,chromatininteractionDOI:10.3724/SP.J.1206.2010.00158*Correspondingauthor.Tel:86-10-87788715,E-mail:yudianking@Received:March29,epted:June7,2010
全文预览
