nov, W. Hu, A.V. Klieve, D. Ouwerkerk, M.A. Sundest, and Y. Kamagata. 2003. Ecology of Uncultivated Oscillospira Species in the Rumen of Cattle, Sheep and Reindeer as Assessed by Microscopy and Molecular ANBIOSCI TECH LTD深圳市安必胜科技有限公司联系电话:0755-8348 9872 传真:0755-8348 9700 email:anbiosci@.comQQ:854520684Approaches. Appl. Environ. Microbiol. 69(11): 6808-6815. 昆虫样品:此类样品可用UltraClean? Tissue & Cells DNA Isolation Kit进行提取。珊瑚礁(Coral Reef)PowerSoil也同样适合提取珊瑚样品中共生的微生物PCR级纯度基因组DNA。从珊瑚礁上取1.3cm直径的珊瑚块,10X TE缓冲液冲洗获得微生物。把沉淀下来的微生物细胞连同缓冲液一起用PowerSoil或Ultraclean Soil提取DNA。下文献有更多操作细节。相关文献:Rohwer, F., M. Breitbart, J. Jara, F. Azam, and N. Knowlton. 2001. Diversity of bacteria associated with the Caribbean coral Montastraea franksi. Coral Reefs 20: 85-91.更多实用技巧请参阅eibo/blog.htm
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