he expression of β-catenin p rotein was increased in the nucleus pulposus of rabbit lumber intervertebral disc degeneration model induced with TNF-α, and there was dose-effec t relationship between them, which demonstrate that canonical Wnt / β-catenin pathway could be do se-effect induced by TNF-α in rabb it lumber intervertebral di sc degeneration model. Key words β-catenin, nucleus pu lposus, Wnt pathway, degeneration,TNF-α. Part Ⅲ Construction of binant adenovirus vector containing human Dickkopf-1 marked by eGFP Objective To construct、verificate and amplificate the adenovirus vector containing human Dick kopf-1 marked by eGFP Methods Primers containing matched s equence and digest site were synthesized according to the Dkk-1 gene order. Dkk-1 cDNA obtai ned 11
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