te through smelting seedling processing is significantly higher than no smelting seedling; ② The length of smelting seedling time has a significant impact on the tube seedlings transplanting survival rate ;③ The exercise processes of closed bottle and open ed bottle are essential, the lack of one stage will cut the transplanting survival rate. (11) When transplanting, different type of matrix have a significant effect on the survival rate of seedlings, seedlings growth also has a big difference. The t est results show ed that the survival rate of peat soil: perlite: river sand = 1:1:1 is significantly higher than other matrix types, reached 83.27%, .The seedlings are robust , leaves is thick green , and growth is best. Key words: Sapindus mukurossi T issue culture Fast propagation in vitro