gakaryocytecolony一formingunit(CFU一MK)wasmeasuredsimultaneously.Theresultsshowedthatafterdefiniteddaysofculture,binationTPO/FL/IL一6/IL?一3wasthemostsuitableforMPBtoobtainhighnumberofMK,andbetterthananyotherthreegroups(P<O.05).TheincreasemultipleofCD4l+cellswas93.97±l7.27onday5andl31,23±l&26onday10.Onday15,theproportionandtheincreasemultipleofCD41+cellsdecreasedobviously.TheexpansionrnultiplesofCFU一MKwere93.33±10.02onday5and120,67±l3.01onday10,higherthananyothergroups.ItisconcludedthatTPO/FL/IL-6/IL-3comb〜nationwasthebestoptimalforexpansionexvivoofmegakaryocyticprogenitorsfromMPB.anditssuitab1edurationofculturewas1Odays:acu1turesystemforexpansionexvivoofmegakaryocyticprogenitorshavebeenestablishedinthisstudy,[Keywords?]mobilizedperipheralblood:megakargocyte;exvivoexpansion作者单位:广东省广州市妇婴医院儿科
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