大肠杆菌耐药株体外诱导其acrA和marA基因分析

2T he amplification of acrA gene with PCR 1: 25922 ; 2:SEMR; 3:SEICI; M: DL2,000 DNA Marker 1: acrA ;2: DL2,000 DNA Marker 图6克隆质粒的酶切鉴定 Figure 6T he identification of Cloning plasmid monitoring with restriction enzyme digestion 1:λ- Hind Ⅲ Marker; 2,3,4: marA - pGEM -T of 25922,SEMR,SEICI;5: DL2,000DNA Marker 图3 marA 基因的 PCR 扩增结果 Figure 3T he amplification of acrA gene with PCR 1: 25922 ; 2:SEMR; 3:SEICI; M: DL2,000 DNA Marker 图1 SEMR 、 SEICI 、 25922 菌株基因组 DNA 提取物的琼脂糖电泳 Figure 1 gelose gel electrophoresis with Genome DNA of 25922 ,SEMR and SEICI. 1:λ- Hind Ⅲ digest DNA Marker; 2: E.Coli 25922 ; 3: SEMR; 4: SEICI 图 5克隆质粒的酶切鉴定 Figure 5T he identification of Cloning plasmid monitoring with restriction enzyme digestion 1 ,2,3: ac rA- pGEM -T of TCC25922,SEMR,SEICI 4:λ- Hind Ⅲ digest DNA Marker;