MaterialDRYING AND GRINDING:?Contains volatile or pounds:snap–freeze, wash, Grind in a mortar with liquid nitrogen?Usual: dry on trays at ambient temperature and in a room with adequate ventilation. dried in an oven; Storage for short periods.2.1.1. Preparation of Plant MaterialDRYING AND GRINDING:Aim of grinding: increasing the surface areafacilitating the ration of solvent into the cells2.1.2. Range of Extraction MethodsMACERATION:?room temperature?using mechanical shakers or mixers to guarantee homogenous mixing?Disadvantage: time-consuming consume large volumes of solvent 2.1.2. Range of Extraction MethodsULTRASOUND-ASSISTED SOLVENT EXTRACTION:?through the production of cavitations?instrument frequency, and length and temperature of sonication?rarely applied to large-scale extraction