dded, the amount of NADPH drastically increased to 80%, due to the cofactor reduction by LbADH. РAfter starting the P450-catalyzed ?hydroxylation by adding Prog, the amount of NADPH dropped to 70%.РMost of the cofactor is present in reduced ?form.РWhen the steroid formation rate decreased ?over the reaction time, the NADPH concentration increased again.РRedox cofactor concentration during biotransformationРPreparative repetitive batch experimentsРThis indicates the limited stability of the biocatalyst.РThis indicates that the stability of the P450 limits the repetitive ?batch in addition to the cofactor supply.Р工作汇报Р1. 发酵培养基中不同种类碳源对菌体生物量的影响Р碳源种类Р葡萄糖Р甘油Р第一批菌体量(g/L)Р17.5Р18.9Р第二批菌体量(g/L)Р18.3Р21Р2. 静息细胞转化实验Р1.葡萄糖?2.甘油?3.甘油+乙醇?4.葡萄糖+0.05VBР5.葡萄糖+0.1VB?6.葡萄糖+5 乙酸钠?7.葡萄糖+10 乙酸钠?8.葡萄糖+15 乙酸钠Р试验组
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