in position, especially N. halophila, Fragilaria capucina, C. perpusilla and Phormidium corium Gom. Theaverage cell density and biomass of periphyton were 1105.5 ind./m2and 2692.0 mg/m2, respectively. Although seasonaland spatial distribution of density and biomass were not significant difference, the overall trend of spatial distributionshowed that the density and biomass in lower-middle region were higher than those in upper region. Average values ofdiversity indices (D, H′), evenness (J) and abundance (Dm) of periphyton were 0.38, 3.04, 0.66 and 2.55, respectively.The water quality of Irtysh River has mild contamination based on the diversity index and phytoplankton abundance.Key words: Periphyton; Community structure; Abundance; Diversity indices; Water quality evaluation; Irtysh River