theresultSindicatedthatinducedTcells.inearlierstageofdevelopmentexpressedbothTCRQBandTCRY8.Inthelaterperiodofinduction,TcellSweremoremature,mostofinducedTcellSexpressedTCRQBmolecule.AlthoughthiSstudyexistedsomedefect,suchasinducedTcelisinvitrocouldnotsecretefunctionalcytokinesafterstimulated,thiSexperimentfirsttimerevealedtheaffectofIX体外诱导小鼠胚胎干细胞分化为T淋巴细胞样细胞thymosinQ1andthymicpolypeptideinjectiononTcellthymicdevelopmentbyaddingexogenouscytokines,thymosin1andthymicpolypeptideonthebasisofEBs.Atthesametime,binationofdifferentcytokinesandthymicpolypeptide.anculturesystemandstromalcell1ine,itprovidesanidealtoolfordetailedexperimentalanalysisofthedifferentiationprogramandanewresourceofclinicalapplicationsofTcells.Keywords:thymosinQ1,thymicpolypeptide,thymopentin,embryonicstemcel1。Tcel1phenotype.X