n-amino acid substrates is similar to the original catalytРР ic mechanism, but further studies (LC-MS/NMR) are needed to clarify the products. However, the catalytic efficiency of these non-ami- РР no acid bound substrates is much lower than that of iV-methylamino acids and, in addition, the catalytic mechanism and products of the РР non-amino acids are not yet known. In our further work, there is a need to further research the catalytic ability for such substrates and to РР purify and characterize the products.РР Key words Thermomicrobium roseum sarcosine oxidase ； site-saturation mutagenesis ； iV-demethylation ； enzymatic properties ； conserved РР residuesРР 2022年第48卷第11期(总第455期)9
全文预览
