基因芯片分析 PPT课件

? 人类、大鼠和小鼠的全基因组芯片。Р基因芯片发展过程РSouthern & Northern ?BlotРDot BlotРMacroarrayРMicroarrayР5РsampleРtargetРhybridizationРlabelРprobeРimageРData analysisР原理-- 通过杂交检测信息Р一组寡核苷酸探针Р—РTATGCAATCTAGРCGTTAGATРACGTTAGAРATACGTTAGATCРTACGTTAGР由杂交位置确定的一组Р核酸探针序列РGTTAGATCР杂交探针组РTATGCAATCTAGР重组的互补序列Р靶序列РTACGTTAGРACGTTAGAРATACGTTAРCGTTAGATРGTTAGATCРATACGTTAРPrototype AmpliOnc™ I BiochipРAmpliOncTM I Biochip after hybridization; posite of red, blue and green imageРThis biochip contains all genomic regions that have been reported to be amplified in cancers.Р1Р2Р3Р4Р5Р6Р7Р8Р9Р10Р11Р12Р13Р14Р15Р16Р17Р18Р19Р20Р21Р22РXРYРOncogene Targets On the AmpliOnc™ I BiochipРPDGFBРEGFR1РPDGFRAРMETРFGFR2РWNT1РMYBРHER2РYES1РHRAS1РCND1РRAF1РGLIРMYCРMDM2Р20q13РRELРMYCL1РFGRРFESРABLРINT2РPIK3CAРNMYCРAKT2РFGFR1РJUNBРAKT1РKRAS2РCDK4РAR