高表达Ang1基因的间充质干细胞株的建立及其肺内转化研究PPT课件

+Ang1MSCsByLentiviralvectorMSCstransducedwithAng1MiceLPSinducedlunginjuryInvivointerventionMethodsandresultspCR2.1-Ang1:Prof.FedericoBussolinoinInstituteforcancerresearchandtreatment,ItalySequencingresults:100%coincidewithAng1cDNAsequenceElectrophoresisaftercuttingbyrestrictionenzymepLVpCMV-dR8.74pMD2GInvitroLentivirus-mediatedAng1/eGFPtransferintoMSCsIsolation,purificationandexpansionofMSCsBonemarrowwasharvestedfrom6weeksoldC57BL/6byflushingthefemursandtibiasSeparateMSCsfrombonemarrowbydensitygradientcentrifugationinFicoll(Pharmacia)CulturedandpassagedwithPeister’smethodPeisterA,MelladJA,LarsonBL,HallBM,GibsonLF,ProckopDJ.Adultstemcellsfrombonemarrow(MSCs)isolatedfromdifferentstrainsofinbredmicevaryinsurfaceepitopes,ratesofproliferation,anddifferentiationpotential.Blood2004;103:1662-1668.