生物化学常用缓冲液

d’s缓冲液的主要优点是不参加和不干扰生物化学反应过程,对酶化学反应等无抑制作用,所以它们专门用于细胞器和极易变性的、对pH敏感的蛋白质和酶的研究工作。其缺点是:?价格昂贵,?对测定蛋白质含量的双缩脲法和Lowry法不适用,因为它们会使空白管的颜色加深。PBS是最普遍不过的实验室缓冲液,但其配方各异。以下是我的总结:母液的配制:0.2MNa2HPO4:称取71.6gNa2HPO4-12H2O,溶于1000ml水0.2MNaH2PO4:称取31.2gNaH2PO4-2H2O,溶于1000ml水各种浓度PB(pH=7.4)的配制:先配0.2MPB(pH=7.4,100ml):取19ml0.2mol/L的NaH2PO4,81ml0.2mol/L的Na2HPO4,即可。然后只需将0.2MPB(pH=7.4)按相应比例适当稀释即可,如:0.1MPB(PH=7.4):取500ml0.2MPB,加水稀释至1000ml即可。0.01MPB(PH=7.4):取50ml0.2MPB,加水稀释至1000ml即可。0.02MPB(PH=7.4):取100ml0.2MPB,加水稀释至1000ml即可。若需要NaCL的话,加入NaCL至0.9%(g/100ml)即可。另另另:其它各种另PH值的0.2MPB(100ml)配方:pH0.2MNaH2PO4(ml)0.2MNa2HPO4(ml)5.793.56.55.89285.990106.087.712.36.185156.281.518.56.377.522.56.473.526.56.568.531.56.662.537.56.756.543.56.851496.945557.038627.133677.228727.323777.419ml81ml7.516847.613877.710.590.57.88.591.57.97938.05.394.7