eРReagent 3 peroxidase labeled chain enzyme - white - white - typeРIntroduction to the experimental procedure:РCell climbing tablets: cold acetone or 4% polyformaldehyde fixed cell crawlsРSet 0.1 mol PBS room temperature for 10 minutesРRepair: add compound digestive juice (0. 125 trypsin % + 0. 01 EDTA %) (0.Р1 molpbs) 4 degrees 30 to 40 minutes 0. 1 mole of PBS wash three timesРEvery minuteРSerum closed: (reagent 1) room temperature for 10 minutesРPour toРA resistance: 37 °C for 1 hour or 4 through the nightР0. 1 mol wash three times 一 three minutes every timeРThe corresponding biotin HuaEr resistance (reagent 2) 37 °C for 30 to 40 minutesР0. 1 mol for three minutesРChain avidin - alkali phosphorus yoke compound enzyme or enzyme avidin peroxidase tag chain (reagent 3) 37 °C for 40 minutes
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