replication ? A reaction in which daughter DNAs are synthesized using the parental DNAs as the template. ? Transferring the ic information to the descendant generation with a high fidelity replication parental DNA daughter DNA Daughter strand synthesis ? Chemical formulation: ? The nature of DNA replication is a series of 3 ′ - 5 ′ phosphodiester bond formation catalyzed by a group of enzymes. Phosphodiester bond formation Template: double stranded DNA Substrate: dNTP Primer: short RNA fragment with a free 3 ′ -OH end Enzyme: DNA-dependent DNA polymerase (DDDP), other enzymes, protein factor DNA replication system Characteristics of replication ? Semi-conservative replication ? Bidirectional replication ? Semi-continuous replication ? High fidelity
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