em43:439–443FukudaK,WatanabeM,AsanoK,UedaH,OhtaS(1990b)Breedingofbrewingyeastproducingalargeamountofβ-phenylethylalcoholandβ-phenylethylacetate.AgricBiolChem54:269–271FukudaK,WatanabeM,AsanoK,OuchiK,TakasawaS(1992)MolecularbreedingofasakeyeastwithamutatedARO4genewhichcausesbothresistancetoo-fluoro-DL-phenylalanineandincreasedproductionofβ-ylalcohol.JFermentBioeng73:366–369附表1原位产物分离技术(ISPR)生产2-苯乙醇本译文习作草稿仅供浏览,错误之处请反馈wangjunr@第7页共8页2005-2006学年研究生植物化学课程译文课程教师王俊儒2006.3.10方法优点缺点细胞是否需要分离参考文献液-液萃取成本低,简便溶剂选择严格否/是Fabre等1996b可大量转移同步分离,则对依据溶剂对细胞的影Stark2001细胞毒性较小。响固定溶剂易处理费用高否Stark2001可大量转移需额外固定CO2超临界萃取可大量转移对细胞有害是Fabre等1999需移出所有疏水性化合物吸附有选择性容量低否/是Klingenberg,Hanssen1988;Fabre等1996Pervaporation有选择性低转移否Bluemke2000产物富集较高无菌Perstraction可大量转移稀释有机相中的否Fabre等1996b产物浓度溶剂选择严格对细胞有毒害本译文习作草稿仅供浏览,错误之处请反馈wangjunr@第8页共8页