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基于厌氧条件下转录组分析的...增李斯特氏菌强启动子的鉴定 汪舒颖

上传者:业精于勤 |  格式:pdf  |  页数:8 |  大小:1224KB

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screened and the greenРРfluorescent protein report strains were constructed. The Pan 4 promoter with the highest regulatory expression level wasРРselected according to the fluorescence intensity of the strain. In addition,the Pan 4 promoter could also successfullyРregulate the expression of azurin and θ toxin,which had a certain broad applicability. This study provided an effectiveРmolecular tool for optimizing the expression and regulation of exogenous proteins in the application of attenuated L.Рmonocytogenes in tumor drugs or vaccine live vectors.РKey words  L. monocytogenes;RNAseq;anaerobic;promoter;green fluorescent proteinРРР —8 —

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