screened and the greenРРfluorescent protein report strains were constructed. The Pan 4 promoter with the highest regulatory expression level wasРРselected according to the fluorescence intensity of the strain. In addition,the Pan 4 promoter could also successfullyРregulate the expression of azurin and θ toxin,which had a certain broad applicability. This study provided an effectiveРmolecular tool for optimizing the expression and regulation of exogenous proteins in the application of attenuated L.Рmonocytogenes in tumor drugs or vaccine live vectors.РKey words L. monocytogenes;RNAseq;anaerobic;promoter;green fluorescent proteinРРР —8 —