ted Affinity Purified Anti-mouse IgG (Rabbit)Р1:10000РRocklandРIRDye 700DX Conjugated Affinity Purified Anti-Rabbit IgG (Donkey)Р1:10000РRocklandРIRDye 800 Conjugated Affinity Purified anti-Rabbit IgG [Donkey]Р1:10000РRocklandРIRDye 700DX Conjugated Affinity Purified Anti-Goat IgG F(c) (Rabbit)Р1:10000РRocklandРIRDye 800 Conjugated Affinity Purified Anti-Goat IgG (Rabbit)Р1:10000РRocklandР 6.引物序列Р基因名称Р用途Р引物序列РPax6РMice genotypingР(F) 5’-GATGAGATGGGAGTTGGGTG-3’Р(R) 5’-AGGTGTGGTGGG-3’.РPax6РPax6Р全长克隆Р(F) 5’-ATAATGCTTCATGCAGAACAGTCACAGCGGAG-3’,Р(R) 5’-AGTACTGAGA-3’Р(include Hind III and Sal I restriction sites).РSox2 promoterРluciferase assayР(F) 5’-ATGG-3’,Р(R) 5’--3’Р(Include Bam H I and Hind III restriction sites).РSox2 promoterРChIPРassayР(F) 5’-ATTTCGAGGGTGCTTGACAAGA-3’,Р(R) 5’-AGGGT-3’.
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