园艺植物生物技术(理论课教案)河南科技学院汉语

inginmoredetail→InducingMutationsPhysicalMutagens(irradiation)Neutrons,AlpharaysDenselyionizing(“Cannonballs”),mostlychromosomeaberrationsGamma,Beta,X-raysSparselyionizing(“Bullets”),chromosomeaberrations&pointmutationsUVradiationNon-ionizing,causepointmutations(ifany),ratingChemicalMutagens(carcinogens)ManydifferentchemicalsMostarehighlytoxic,usuallyresultinpointmutations(二)组织培养过程变异的利弊与利用AdvantagesScreenveryhighpopulations(cellbased)CanapplyselectiontosinglecellsDisadvantagesManymutationsarenon-heritableRequiresdominantmutation(ordoublerecessivemutation);mostmutationsarerecessiveCanavoidthisconstraintbynotapplyingselectionpressureinculture,butyouloosetheadvantageofhighthrough-putscreening–havetogrowoutallregeneratedplants,produceseed,andevaluatetheM2Alternative:essusingSomaclonalVariation