sequence ( location in GenBank as AJ575250 ) showed 98.8% homologies in nucleotide sequence and 100% in amino acid sequence respectively. 4. Cloning of PsENOD40 gene The PsENOD40 gene fragment was amplified by meas of PCR using pea genomic DNA as template and a pair of specific oligonucleotides at5’- and 3’- end as primer. Sequence analysis showed that the fragment consisted of 459 bps, which contains contained an open reading frame of 228 bps, and encoded a polypeptide of 75 amino acids. The iniation codon of this gene was not ATG but TTA. Comparison with previously published sequence ( location in GenBank as X81064 ) showed 100 % homologies in nucleotide sequence and 100% in amino acid sequence respectively. Keywords : Pea ; Pslectin ; Early nodulin gene ; PsENOD12A ; PsSYM10 ; PsENOD4 0
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