介观流控-阀上实验室系统光度法定量分析蛋白质的研究

calchangesandstructuraltransformationoftheaimanalyteareusuallyunavoidableduetotheintroducingofwashingsolutioneluenttoobtainsatisfactoryrecovery,whicheventuallycontributedtoadeterioratedprecision.Thein-situdetectiontechnique,suchasthesolid—phasesurfacefluorescence,offersanexcellentalternativetothequestionsmentionedabove.Inthesecondpartofthethesis,apreliminarystudyforproteinassayWasperformedwithsolid—surfacefluorescencetechniquebyexploitingIII东北大学硕士学位论文AbstractsilicagelassolidphasematerialandOPAasfluorescenceprobe.Undertheoptimalconditions,alinearcalibrationcurvewasobtainedintherangeof1—200lagml一,alongwithadetectionlimit(30)of0.56lagml~.Thismethodologyprovidesapracticalprotocolfortheon-ics.Keywords:Meso--fluidiclab·-on·-valvesystem;protein;Congored;spectrophotometry;solid.surfacefluorescence