0.05%Tween-20) for 1h at room temperature.\rThe membrane was then incubated with monoclonal antibody of mouse anti human ezrin\rp81(Maixin-Bio) followed by reaction with anti-mouse IgG-HRP antibody. Reactive protein was\rdetected by ECL chemiluminescence system (Santa Cruz).\rezrin 蛋白表达通过 Western blot 进行检测。已达融合生长的单层细胞用冷 PBS 洗三次后，\r加入裂解液(50mM Tris-HCl, pH8.0,150mM NaCl, 100μg/ml PMSF, 1%TritonX-100)冰上裂\r解 30min，12,000g,5 min 离心去除细胞碎片后，取 50μg 样品与上样缓冲液混合，煮沸 5min，\r进行 10% SDS-PAGE 电泳，转硝酸纤维素膜(Pall Corporation)。将膜在含 5%脱脂奶粉的\rTBST(10mM Tris-HCl, pH7.5,150mM NaCl, 0.05%Tween-20)中室温下封闭 1h，随后加入鼠抗\r人 ezrin 一抗(Maixin-Bio)，抗鼠 IgG-HRP 二抗，ECL 化学发光试剂检测(Santa Cruz)。\r电话：021-51320182 0571-56807986 E-mail:river@liferiver.com.cn
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